AlamarBlue®

PRODUCT DESCRIPTION

AlamarBlue® is a highly sensitive resazurin-based cell viability reagent designed for rapid assessment of cellular metabolic activity, proliferation, cytotoxicity, and drug sensitivity in biological systems.

The reagent operates through reduction of the non-fluorescent blue dye resazurin into the highly fluorescent pink compound resorufin by metabolically active living cells.

This provides a simple, non-radioactive, and highly reproducible assay system widely used in:

• Cell biology
• Cancer research
• Toxicology
• Drug discovery
• Microbiology
• Stem cell research
• Tissue engineering

SCIENTIFIC PRINCIPLE

Redox Conversion Mechanism

Oxidized Form (Blue)

Resazurin

↓

Reduced Form (Pink/Fluorescent)

Resorufin

Metabolically active cells reduce resazurin through mitochondrial, cytosolic, and microsomal enzymes, generating measurable colorimetric and fluorescent signals proportional to viable cell number.

DETECTION METHODS

APPLICATIONS

Cell Biology

• Cell proliferation assays
• Cell viability analysis
• Cell metabolic activity studies
• Cytotoxicity testing

Cancer Research

• Anticancer drug screening
• Chemotherapy sensitivity testing
• Tumor cell metabolic profiling

Pharmaceutical Research

• Drug discovery screening
• High-throughput screening (HTS)
• Toxicological studies

Microbiology

• Microbial viability assessment
• Antimicrobial susceptibility testing

Biotechnology

• Bioprocess monitoring
• Cell culture optimization

FEATURES & ADVANTAGES

TYPICAL WORKFLOW

1. Seed cells into microplate
2. Add treatment/drug
3. Incubate cells
4. Add AlamarBlue® reagent
5. Incubate for signal development
6. Measure absorbance or fluorescence
7. Analyze viability data

COMPATIBLE SYSTEMS

• 96-well plates
• 384-well plates
• Fluorescence plate readers
• UV-Visible microplate readers
• Cell culture incubators

SAFETY INFORMATION

• For laboratory research use only
• Avoid ingestion and inhalation
• Wear gloves and eye protection
• Avoid microbial contamination
• Handle under aseptic conditions

ASSOCIATED LAB PRODUCTS

• DMEM Media
• RPMI-1640
• PBS Buffer
• Trypsin-EDTA
• CO₂ Incubator
• Cell Culture Plates
• Micropipettes
• Fluorescence Plate Reader

TARGET USERS

• Cell Biology Laboratories
• Pharmaceutical Research Labs
• Biotechnology Companies
• Cancer Research Institutes
• Universities & Research Institutes
• Molecular Biology Laboratories
• Toxicology Laboratories

WLS QUALITY NOTE

This reagent is ideal for advanced viability and metabolic assays requiring sensitive, reproducible, and non-destructive cellular analysis across biomedical, pharmaceutical, and biotechnology applications.