Glycine – NaOH Buffer

Glycine–Sodium Hydroxide Buffer Solution (Glycine–NaOH Buffer)

Product Description:

Glycine–NaOH buffer is an alkaline buffer system prepared from glycine (amino acid) and sodium hydroxide, widely used in biochemical and analytical applications. It provides stable pH conditions typically in the alkaline range (pH 8.6–10.6), making it suitable for protein electrophoresis, enzyme assays, and biochemical reactions requiring controlled pH environments.

Chemical Composition:

Glycine (NH₂CH₂COOH)

CAS No: 56-40-6

Sodium Hydroxide (NaOH)

CAS No: 1310-73-2

Purified Water

Typical Specifications:

Appearance: Clear, colorless solution

pH Range: 8.6 – 10.6 (depending on formulation)

Solubility: Completely miscible in water

Buffer Type: Amino acid-based alkaline buffer

Grade: Technical / Laboratory Grade

Applications:

Buffer system for protein electrophoresis (e.g., SDS-PAGE running buffer)

Enzyme activity studies and biochemical assays

pH stabilization in laboratory reactions

Used in chromatography and molecular biology protocols

Suitable for research and educational laboratory use

Handling & Safety Information:

Contains sodium hydroxide – corrosive

Avoid contact with skin, eyes, and clothing

Use appropriate PPE: gloves, goggles, lab coat

Do not ingest or inhale vapors

Handle in a well-ventilated laboratory environment

Refer to Safety Data Sheet (SDS) before use

Storage Conditions:

Store at 10–25°C

Keep in tightly closed amber bottle

Protect from light and contamination

Avoid prolonged exposure to air (CO₂ absorption may alter pH)

Hazard Information:

Hazard Class: Corrosive (due to NaOH content)

May cause severe skin burns and eye damage

Not classified as flammable

Packaging:

1000 mL amber reagent bottle (standard WLS packaging)

Other pack sizes available upon request.