COOMASSIE BRILLIANT BLUE G-250

COOMASSIE BRILLIANT BLUE G-250 – AR GRADE

Chemical Name: Coomassie Brilliant Blue G-250
Synonym: Acid Blue 90
Molecular Formula: C₄₇H₄₈N₃NaO₇S₂
Molecular Weight: 854.02 g/mol
CAS Number: 6104-58-1
Grade: AR (Analytical Reagent)
Form: Dye powder
Pack Size: 5 g
Packaging: Amber/HDPE reagent bottle

Description
Coomassie Brilliant Blue G-250 is a triphenylmethane dye widely used in biochemical and analytical laboratories. It is highly valued for its strong binding affinity to proteins, forming a stable blue complex used in quantitative protein assays.

Specifications (Typical AR Grade)
Dye Content: ≥ 95%
Appearance: Dark blue to reddish-brown powder
Solubility: Soluble in water and alcohol (forms deep blue solution)
Absorption Maximum: ~595 nm (protein-bound form)

Applications
Bradford Protein Assay (standard use)
Protein quantification in biochemistry labs
Gel electrophoresis staining (proteins)
Spectrophotometric analysis
Analytical dye applications

Handling & Storage
Store in a cool, dry place
Keep container tightly closed
Protect from moisture and light
Avoid dust formation

Hazard Information
May cause skin and eye irritation
Harmful if swallowed in large amounts
Dust may cause respiratory irritation

Safety Precautions
Use gloves, goggles, and lab coat
Avoid inhalation of powder
Handle in well-ventilated area

Packaging
Standard: 5 g bottle (as requested)
Also available: 1 g, 10 g, 25 g
Comparable Suppliers
Sigma-Aldrich
Merck
SRL
Loba Chemie
HiMedia
WLS Safety Note
⚠️ For laboratory use only. Not for human consumption. Handle with standard laboratory safety precautions.