4-Nitrophenyl-β-D-Glucopyranoside (pNPG)

4-Nitrophenyl-β-D-Glucopyranoside

COMMON NAME
pNPG

CHEMICAL NAME
4-Nitrophenyl-beta-D-glucopyranoside

PRODUCT CODE
70410

PRODUCT CATEGORY
Biochemical Reagent / Enzyme Substrate

GRADE
Laboratory Grade / Analytical Grade

APPEARANCE
White to off-white crystalline powder

MOLECULAR FORMULA
C12H15NO8

MOLECULAR WEIGHT
301.25 g/mol

CAS NUMBER
2492-87-7

ASSAY / PURITY
≥98%

PACK SIZE
1 g
(Other pack sizes may be available)

MANUFACTURER
Molychem

APPLICATIONS
• β-Glucosidase activity assay
• Enzyme kinetics studies
• Glycosidase substrate analysis
• Microbiology and biotechnology research
• Biochemical pathway investigations
• Pharmaceutical and enzymology research

PRINCIPLE OF USE
pNPG acts as a chromogenic substrate for β-glucosidase enzymes. Hydrolysis releases p-nitrophenol, producing a yellow color under alkaline conditions that can be measured spectrophotometrically.

SOLUBILITY
Soluble in water and methanol

COMMON DETECTION WAVELENGTH
405 nm

ANALYTICAL TECHNIQUE
UV-Visible Spectrophotometry

RECOMMENDED STORAGE
Store at 2 – 8 °C
Protect from moisture, light, and heat.

STABILITY
Stable under recommended storage conditions.

HAZARD INFORMATION
• For laboratory and research use only
• Avoid inhalation and direct contact
• Use appropriate PPE during handling
• Keep container tightly closed after use

SAFETY PRECAUTIONS
• Wear gloves, laboratory coat, and safety goggles
• Handle in a well-ventilated environment
• Avoid ingestion or inhalation
• Wash hands thoroughly after handling

TARGET USERS
• Research Laboratories
• Universities and Institutes
• Biotechnology Laboratories
• Pharmaceutical Laboratories
• Biochemistry Researchers
• Microbiology Laboratories

ASSOCIATED REAGENTS
• Sodium Carbonate
• Phosphate Buffer
• β-Glucosidase Enzyme
• Distilled Water
• Standard p-Nitrophenol Solution

PACKAGING
Packed in sealed amber reagent bottle with protective carton packaging.

WLS QUALITY NOTE
This reagent is suitable for sensitive enzymatic and biochemical assays requiring reliable chromogenic substrate performance and spectrophotometric detection.

SUPPLIED BY
WinLabs & Stores (WLS)
Email: info@labstores.ng
Phone: +2348059999930