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4-Nitrophenyl-β-D-Glucopyranoside
pNPG
Analytical Reagent
1g
4-Nitrophenyl-β-D-Glucopyranoside
COMMON NAME
pNPG
CHEMICAL NAME
4-Nitrophenyl-beta-D-glucopyranoside
PRODUCT CODE
70410
PRODUCT CATEGORY
Biochemical Reagent / Enzyme Substrate
GRADE
Laboratory Grade / Analytical Grade
APPEARANCE
White to off-white crystalline powder
MOLECULAR FORMULA
C12H15NO8
MOLECULAR WEIGHT
301.25 g/mol
CAS NUMBER
2492-87-7
ASSAY / PURITY
≥98%
PACK SIZE
1 g
(Other pack sizes may be available)
MANUFACTURER
Molychem
APPLICATIONS
• β-Glucosidase activity assay
• Enzyme kinetics studies
• Glycosidase substrate analysis
• Microbiology and biotechnology research
• Biochemical pathway investigations
• Pharmaceutical and enzymology research
PRINCIPLE OF USE
pNPG acts as a chromogenic substrate for β-glucosidase enzymes. Hydrolysis releases p-nitrophenol, producing a yellow color under alkaline conditions that can be measured spectrophotometrically.
SOLUBILITY
Soluble in water and methanol
COMMON DETECTION WAVELENGTH
405 nm
ANALYTICAL TECHNIQUE
UV-Visible Spectrophotometry
RECOMMENDED STORAGE
Store at 2 – 8 °C
Protect from moisture, light, and heat.
STABILITY
Stable under recommended storage conditions.
HAZARD INFORMATION
• For laboratory and research use only
• Avoid inhalation and direct contact
• Use appropriate PPE during handling
• Keep container tightly closed after use
SAFETY PRECAUTIONS
• Wear gloves, laboratory coat, and safety goggles
• Handle in a well-ventilated environment
• Avoid ingestion or inhalation
• Wash hands thoroughly after handling
TARGET USERS
• Research Laboratories
• Universities and Institutes
• Biotechnology Laboratories
• Pharmaceutical Laboratories
• Biochemistry Researchers
• Microbiology Laboratories
ASSOCIATED REAGENTS
• Sodium Carbonate
• Phosphate Buffer
• β-Glucosidase Enzyme
• Distilled Water
• Standard p-Nitrophenol Solution
PACKAGING
Packed in sealed amber reagent bottle with protective carton packaging.
WLS QUALITY NOTE
This reagent is suitable for sensitive enzymatic and biochemical assays requiring reliable chromogenic substrate performance and spectrophotometric detection.
SUPPLIED BY
WinLabs & Stores (WLS)
Email: info@labstores.ng
Phone: +2348059999930
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