Product Name
N-(1-Naphthyl)ethylenediamine Dihydrochloride (NED)

Chemical Identity

• IUPAC Name: N-(Naphthalen-1-yl)ethane-1,2-diamine dihydrochloride
• Common Name: NED
• Molecular Formula: C₁₂H₁₄N₂·2HCl
• Molecular Weight: 259.18 g/mol
• CAS Number: 1465-25-4
• EC Number: 215-981-2

Grade & Compliance

• Grade: Analytical Reagent (AR) / Laboratory Reagent (LR)
• Purity: ≥ 98%
• Application Standard: Suitable for spectrophotometric analytical procedures
• Common Use Standard: Griess reaction and nitrite/nitric oxide determination

Physical & Chemical Properties

• Appearance: White to light pink crystalline powder
• Odour: Odourless
• Solubility: Soluble in water and dilute acidic media
• Stability: Light-sensitive compound
• pH Sensitivity: Performs optimally in acidic conditions
• Melting Point: ~215–220 °C (decomposes)

Specification (Typical Laboratory Limits)

• Assay: ≥ 98%
• Loss on Drying: ≤ 1.0%
• Sulphated Ash: ≤ 0.1%
• Heavy Metals: ≤ 0.001%
• Insoluble Matter: Passes test

Functional Uses (Laboratory Relevance)

• Chromogenic coupling reagent in Griess assay
• Nitric oxide (NO) quantification studies
• Nitrite determination in biological samples
• Environmental water nitrite analysis
• Biomedical oxidative stress research
• Spectrophotometric color development reagent

Analytical Principle

NED reacts with diazotized sulfanilamide under acidic conditions to form a stable pink azo dye measurable spectrophotometrically.

The chromophore intensity is proportional to nitrite concentration.

Typical absorbance measurement:

• 540 nm
• 543 nm
• 550 nm (method dependent)

Preparation Insight (Lab Use)

Typical preparation for Griess reagent:

• NED dissolved in distilled water or phosphoric acid medium
• Common concentration:
  • 0.1% w/v NED solution

Usually paired with:

• Sulfanilamide
• Phosphoric Acid

Packaging & Presentation

• Container Type: Amber glass or HDPE reagent bottle
• Closure: Chemical-resistant screw cap
• Typical Pack Sizes:
  • 1 g
  • 5 g
  • 25 g
  • 100 g

Hazard & Safety Information

• GHS Classification: Irritant
• Potential Risks:
  • Eye irritation
  • Skin irritation
  • Harmful if swallowed in large quantities
• Precautions:
  • Avoid direct inhalation of dust
  • Use gloves and laboratory eye protection
  • Protect from direct light exposure

Storage Conditions

• Store in a cool, dry, dark environment
• Protect from light and moisture
• Keep container tightly sealed
• Recommended storage temperature:
  • 15–25 °C

Shelf Life

• Typically 2–5 years under proper storage conditions

Manufacturer (Typical Suppliers)

• Loba Chemie Pvt. Ltd.
• HiMedia Laboratories
• Sigma-Aldrich
• SRL Chemicals

Quality Notes (WLS Insight)

• Highly important reagent in nitric oxide estimation protocols
• Sensitive to oxidation and light exposure; amber packaging preferred
• Produces highly stable pink azo chromophore with excellent spectrophotometric sensitivity
• Widely used in biomedical, environmental, and pharmaceutical analysis laboratories

Common Associated Reagents

• Sulfanilamide
• Phosphoric Acid
• Sodium Nitrite Standard
• Griess Reagent Components

Common Biomedical Applications

• Oxidative stress studies
• Inflammation research
• Nitric oxide metabolite quantification
• Antioxidant assay systems
• Cell culture nitric oxide studies
• Plasma and serum nitrite estimation